异丙酚降压作用与ATP敏感性钾通道的关系

The relation between the effect of propofol in decreasing blood pressure and ATP sensitive K+ channels

目的观察异丙酚对高血压犬血流动力学和肾素的影响,并探讨其与ATP敏感性钾通道(KATP通道)的关系。方法雄性健康杂种犬30只,体重(15±5)kg,建立肾动脉狭窄性高血压犬模型后,随即分为3组,分别为对照组、异丙酚组和格列苯脲组。对照组和异丙酚组静脉各给予50 g/L葡萄糖10 mL,格列苯脲组给予格列苯脲10 mL(0.3mg/kg,溶于50 g/L葡萄糖);10 min后对照组给予50 g/L葡萄糖10 mL,其余2组各给予异丙酚10 mL(2 mg/kg,50 g/L葡萄糖稀释)。记录基础、给药后2、10、203、0 min时的平均动脉压(MAP)、心率(HR)、呼吸频率(RR)和肾素活性(PRA)。结果异丙酚组动物血压、心率随时间呈进行性下降(P<0.05),格列苯脲组血压下降趋势弱于异丙酚组(P<0.05),心率的下降趋势则较异丙酚组明显(P<0.05);同时异丙酚组动物肾素活性亦下降(P<0.05),且低于格列苯脲组(P<0.05)。结论异丙酚降压作用与其作用于KATP通道、抑制肾素活性,进而抑制肾素-血管紧张素-醛固酮系统有关。

Objective To observe the influence of propofol on hemodynamics and plasma rennin activity （PRA） in hypertensive dogs, and investigate the relation between propofol and ATP sensitive K4 channels （KATP channels）. Methods Thirty healthy male bastard dogs, weighing （15 ± 5）kg, after establishing renovascular hypertensive canine models, were randomly divided into control group, propofol group and glibenclamide group. The dogs in control group and propofol group were given 10 mL glucose （50 g/L） via venous injection, and the dogs in glibenclamide group were given glibenclamide [0.3 mg/kg, dissolved into 10 mL glucose （50 g/L）] via venous injection. Ten minutes later, the dogs in control group were given 10 mL glucose （50 g/L）, and the dogs in propofol group and glibenclamide group were given 10 mL propofol （2 mg/kg, dissolved in 50 g/L glucose）. The mean arterial pressure （MAP）, heart rate （HR）, respiration rate （RR） and plasma renin activity （PRA） were detected before and 2, 10, 20, 30 rain after administration. Results In propofol group, MAP, HR and PRA decreased gradually （P〈0.05）, whereas in glibenclamide group, the downtrend of MAP and PRA was weaker than that in propofol group （P〈0.05）, and the downtrend of HR was more obvious than that in propofol group （P〈0.05）. Conclusion Propofol could decrease blood pressure, which may be due to its effect on the ATP sensitive KATe channels which could inhibit plasma renin activity （PRA） and RAAS.