高氟对小鼠磨牙牙胚发育的影响

Effect of high-dose fluoride on tooth germ development of mouse molar

目的探讨高氟环境对小鼠磨牙牙胚发育的影响。方法建立小鼠急性氟中毒动物模型,采用HE染色方法,观察高氟环境下颌第一磨牙牙胚发育的蕾状期、帽状期、钟状形态发生期、分化期、分泌期、成熟期成釉细胞形态的变化,并同时观察正常对照小鼠的牙胚发育情况。结果对照组磨牙发育为钟状期时,实验组仍处于帽状期。实验组钟状分化、分泌期成釉细胞细胞变矮,部分细胞极性消失,细胞排列紊乱。结论过量氟的摄入可使磨牙发育明显延迟。过量氟对钟状分化、分泌期成釉细胞的细胞形态有较强影响。

Objective To study the tooth germ development of mouse molar in fluorosis. Methods We established an acute fluorosis model and used HE-staining after preparing specimens of different stage of mouse first molar developing tooth germ. Results The developing molars of the control fetuses were at the bell stage of odontogenesis, whereas those of the experimental population were at cap stage. The ameloblasts became shorter and lost polarity. The arrangement of ameloblasts fell into disorder at the bell of differeiational stage and secretory stage. Conclusion The developing molars of the experimental fetuses were retarded. High-dose fluoride has a strong effect in the ameloblasts at the bell of differetiational stage and secretory stage.