应用甲状腺素片及饥饱失常法建立肝郁证大鼠模型的研究

Study on the rat model of liver-depression syndrome established by thyroxine tablet and irregularity of food

目的探讨建立中医肝郁证大鼠模型的一种新的造模方法。方法依次采用甲状腺素片灌胃、饥饱失常法喂养,观察大鼠的行为学变化,测量大鼠肛温、并用夹心ELISA法检测其血浆五羟色胺(5-hydroxytrypta mine,5-HT)、去甲肾上腺素(norepinephrine,NE)、肾上腺素(epinephrine,E)水平的变化及逍遥散对其含量的影响。结果肝郁模型组及逍遥散组大鼠于造模第28天均出现了典型的心肝火旺之症,体温均升高,与空白对照组相比有显著性差异(P<0.05);于造模第44天,肝郁模型组大鼠血浆5-HT、NE、E水平均较前明显升高(P<0.05或P<0.01);且NE、E水平高于逍遥散组(P<0.01和P<0.05);于造模第56天肝郁模型组出现典型的肝郁证的行为学特点,逍遥散组大鼠血浆中5-HT水平低于肝郁模型组(P<0.01)。结论应用甲状腺素片及饥饱失常法建立的肝郁证大鼠模型基本符合中医肝郁证的特征。

Objective To study a new method of establishing the rat model of liver depression syndrome. Methods We adopted gaster-poured with thyroxine tablet and fully-poorly feeding food in turn, observed the praxiological changes of rats, measured the temperature in anus, chose the sandwich ELISA to detect the level change of 5-hydroxytryptamine （5-HT）, norepinephrine （NE）, epinephrine （E） in plasma and the effect of Xiaoyao Powder on change of their contents. Results From the 28th day after establishing the model, the rats both in the liver-depressed model group and the Xiaoyao Powder group showed typical symptoms of heat in the heart and liver. Compared with the blank control group, the temperature was increased significantly （P〈0.05）; at the 44th day of model establishment, in the liver-depressed model group, the level of 5-HT, NE and E in rat plasma was higher than before （P〈0.05 or P〈0.01）, and the level of NE and E was lower than in the Xiaoyao Powder group （P〈0.01 and P〈0.05）. At the 56th day, the typical characteristics of liver-depression syndrome appeared in the liver-depressed model group. The level of 5-HT in the Xiaoyao Powder group was lowerer than in the liver-depressed model group （P〈0.01）. Conclusion The rat model of liver-depression syndrome has been initially established by the method of using gaster-poured with thyroxine tablet and irregularity of food, which is in compliance with the characteristics of liver-depression syndrome.