壳聚糖固定木瓜蛋白酶研究

Study of Immobilization of Papain with Chitosan

木瓜蛋白酶作为外用药，可水解坏死组织，但有副作用。固定化木瓜蛋白酶涂膜剂是由虾壳中提取的壳聚糖与戊二醛交联、固定化制备出来的。０．４％的戊二醛与１％中等粘度壳聚糖（η＝１００～２５０ＭＰａ·ｓ）经摇床振荡（２００ｒ／ｍｉｎ，２ｈ）充分交联，加木瓜蛋白酶液（２．０９ｍｇ／ｍＬ，ｐＨ７．８）４℃固定１２ｈ，涂抹皮肤自然成膜。此固定化涂膜剂最适温度５５℃，最适ｐＨ７．８，最大载酶量为４５ｍｇ／ｇ载体，酶相对活力３０～３５％。与未固定化木瓜蛋白溶液酶相比，提高了其对热、乙醇、脲的稳定性。

Papain is remedy for zymolsising decayed organization,but it has some byeffect. Papain can be immobilized on chitosan and crosslinked with glutaraldehyde: shaking 1% chitosan (η=100～250MPa·s) with 0.4% solution of glutaraldehyde for two hours with a shaketable (200r/min). If 2.09mg/ml papain, pH 7.8 is added and immobilized on the chitosan at 4℃ for 12 hours, papain can be smeared over skin into membrane. The immobiliz ed papain has optimum temperature and optimum pH 55℃ and 7.8 respectively. The maximum load of enzyme is 45mg/g chitosan. Engzyme activity retention is 30～35%. stability of immobilized papain to temperature,alcohol,urea is increased obviously comparred to that of soluble papain. Papain is immobilized on chitosan in order to improve some byeffect of soluble papain. 