Livin靶向siRNA对结肠癌细胞的抑制作用

Inhibitory Effect of Targeted Livin siRNA on the Growth of Human Colon Cancer Cells

目的构建Livin靶向si RNA重组表达载体,研究其对人结肠癌细胞生长的抑制作用。方法构建Livin靶向si RNA重组表达载体并转染结肠癌细胞,通过RT-PCR和Western blot方法检测Livin的表达。结果经酶切鉴定和测序结果证实Livin靶向si RNA重组表达载体构建成功,它对结肠癌细胞Livin mRNA和蛋白的表达抑制率分别为30.18%和28.88%。结论Livin靶向si RNA重组表达载体构建成功并能显著抑制Livin基因的表达。

Objective To construct siRNA recombinant expression vector targeting Livin gene and study its inhibitory effect on growth of human colon cancer cells. Methods siRNA recombinant expression vector targeting Livin gene was constructed and transfected into human colon cancer cells. The effect of siRNA recombinant expression vector was detected by RTPCR and Western blot. Results It was confirmed by restriction endonuclease and sequence analysis that siRNA recombinant expression vector targeting Livin gene was constructed successfully. Inhibition ratio of Livin siRNA at mRNA and protein levels was 30.18% and 28.88% respectively. Conclusion The siRNA recombinant expression vector targeting Livin gene has been constructed successfully and can inhibit the expression of Livin gene in human colon cancer cells significantly.