广西壮族不同程度慢乙肝与HLA-DQA1基因相关性研究

Study on the association of the HLA-DQA1 alleles with the different chronic hepatitis B of the Zhuang nationality in Guangxi Zhuang Autonomous Region

目的探讨广西壮族不同程度慢性乙型病毒性肝炎（慢乙肝）的HLA-DQA1等位基因的相关性。方法采用序列特异性引物-聚合酶链式反应（ssp-PCR）法检测壮族轻、中、重度各30例慢乙肝患者HLA-DQA1等位点并进行基因分析研究。结果轻度、中度组HLA-DQA1^＊0103等位基因的频率较重度组明显升高;重度组HLA-DQA1^＊0301等位基因的频率较慢乙肝轻度组明显降低。结论携带DQA1^＊0103等位基因个体感染HBV以后更易发展为轻、中度肝炎,而DQA1^＊0301可能为重度慢乙肝抗性基因。

Objective To explore the association of the HLA-DQA1 alleles with the different chronic hepatitis B of the Zhuang nationality in Guangxi. Methods In thirty patients with mild , moderate and severe chronic hepatitis B in Zhuang nationality in Guangxi the HLA- DQA1 alleles were identified by using the polymerase chain reaction-sequence specific primers（PCR-ssp） technique. Results The alleles gene frequencies of HLA DQA1^＊ 0103 in the Zhuang patients with mild and moderate chronic hepatitis B was singnificantly higher than that in the patients with severe chronic hepatitis B;HLA- DQA1^＊ 0301 in the patients with severe chronic hepatitis B was lower than that in the patients with mild and moderate＇ s chronic hepatitis B. Conclusion So DQA1^＊-0301 may be protective alleles of severe chronic hepatitis B in Zhuang nationality.