隐丹参酮等中药单体对SZ95细胞内脂质合成的影响

Effects of cryptotanshinone and other traditional chinese medicines on the lipid synthesis of SZ95 sebocytes in vitro

目的观察隐丹参酮等中药单体对SZ95细胞中脂质合成的影响。方法用尼罗河红荧光染色结合流式细胞术,以异维A酸为对照,观察隐丹参酮、丹参酮II-Α、盐酸小檗碱、黄芩甙、苦参碱作用SZ95细胞48h后,1×10^4个尼罗河红染色的SZ95细胞中平均每个细胞的荧光强度变化。结果1.25×10^-7Μ丹参酮II-Α使尼罗河红染色荧光强度下降最为明显（-34.3%,P〈0.05）,其次为1×10^-4Μ黄芩甙（-30.1%,P〈0.05）、1×10^-7Μ异维A酸（-21.0%,P〈0.05）、1.25×10^-7Μ隐丹参酮（-16.2%）、1×10-6Μ盐酸小檗碱（-7.8%）也有抑制作用,但没有统计学差异。1×10^-3Μ苦参碱则显示了促进脂质合成的作用（26.9%）。结论丹参酮II-Α、黄芩甙及异维A酸可能具有直接抑制皮脂腺细胞中脂质合成的作用。

Objective To investigate the effects of Chinese medicines cryptotanshinone, tanshinone II- A, berberine, baicalin,matrine on the lipid synthesis of SZ95 sebocytes ccrnpared with isotretinoin. Metals Lipid contents in the SZ95 sebocytes were labeled with a fluorescence dye（Nile red） and analyzed using flow cytometry. Results Lipid synthesis was inhibited by 1.25 × 10^-7M tanshinone II - A（ - 34.2% ）, 1×10^4 M baicalin（ - 30.1% ）, 1 × 10^-7 Misotretinoin（ 21.1%）, 1.25×10-7MCryptotanshinone（-16.2%）andl×10-5MBerberine（-7.8%）also decreased lipogenesis but no statistical significance was found. Matrine increased lipogenesis（26.913% ）. Conclusion Tanshinone II-A and baicalin may have a direct effect on lipid synthesis in sebocytes.