模拟体液中羟基磷灰石-壳聚糖矿化行为的压电阻抗

Biomineralization of hydroxyapatite-chitosan composite in a simulated body fluid using piezoelectric quartz crystal impedance

采用压电石英晶体阻抗技术(PQCI)研究复合材料羟基磷灰石-壳聚糖(HA-CS)在模拟体液中的矿化行为。研究结果表明:HA-CS在模拟体液中的矿化过程导致石英晶体传感器频率显著降低,当复合材料中HA与CS的质量比为6:4时,矿化能力最强;PQCI实时在线监测所得的频率、动态电阻、动态电感、动态电容和静态电容等参数的变化可用于评价矿化过程中HA-CS复合材料的微观变化;根据矿化过程中频率与动态电阻的变化可将HA-CS在模拟体液中的矿化过程分成3个阶段,并获得各阶段的动力学方程。此研究方法为骨组织工程材料的制备提供了一种新的实时监测技术。

The biomineralization process of hydroxyapatite-chitosan （HA-CS） in a simulated body fluid was studied using piezoelectric quartz crystal impedance （PQCI）. The results show that the frequency decreases obviously during the biomineralization process of hydroxyapatite-chitosan composite and the optimal mass ratio of HA to CS is 6：4. Several parameters （frequency △f, resistance ARm, inductance △Lm, static capacity Cm, dynamic capacity △Cs） are simultaneously obtained from the PQCI on-line measuring and applied to investigating the change of HA-CS during the biomineralization. It is shown the mineralization process of HA-CS can he divided into three steps according to the slope of △f vs. △Rm. The correlative kinetic equations and parameters are obtained from the frequency change. The proposed method can be used in mineralization studies of materials for bone tissue engineering for its advantages in providing real-time multidimensional information.