实时荧光定量RT-PCR检测肝癌组织中miR-122a及miR-224的表达

Detection of miR-122a and miR-224 expression in hepatocellular carcinoma by real-time fluorescence quantitive RT-PCR

目的运用实时荧光定量RT-PCR方法分析miR-122与miR-224两种miRNAs在原发性肝细胞癌(HCC)中的表达,探讨以miRNAs为靶点在早期肝癌中的临床诊断意义。方法采用基于2(-△△CT)的实时荧光定量RT-PCR法对35例肝癌组织及癌旁组织的miR-122a及miR-224进行了定量分析,结果经由Northernblot验证。结果与正常组织相比,在所有的被检肝癌组织中,均发现了miR-122a有不同程度的表达下调(P<0.01),而上述肝癌组织中的miR-224的定量结果显示该miRNA表达显著增加(P<0.001)。结论HCC组织中存在上述两种miRNAs表达水平的改变,实时荧光定量RT-PCR法为早期、准确的在临床上检测肝组织癌变提供了新的思路。

Objective To detect the expressions ofmiR-122 and miR-224 in hepatocellular carcinoma （HCC） by real-time fluorescence quantitative RT-PCR and investigate the significance of miRNAs in early diagnosis of HCC. Methods 2^-△ACT method was used for quantitative analysis of the expression pattern of miR-122 and miR-224 in 35 HCC and adjacent normal tissues. All the quantitative results were confirmed by Northern blotting. Results Compared with adjacent normal tissues, the HCC tissues showed significant miR-122 down-regulation （P〈0.01） and miR-224 over-expression （P〈0.001）. Conclusion HCC has obvious alteration in the expression patterns of miR-122 and miR-224, and real-time fluorescence quantitative RT-PCR provide a new means for early, efficient, and accurate diagnosis of HCC.