减毒耐药变形杆菌对慢性乙型肝炎患者外周血树突细胞CD80、CD86表达的影响

Effect of the drug-resistant attenuated Bacillus proteus on CD80 and CD86 protein expression in peripheral blood dendritic cells of hepatitis B patients

目的观察减毒耐药变形杆菌对慢性乙型肝炎(HBV)患者和健康人DC细胞表面表达的CD80、CD86的影响。方法分离慢性HBV患者和健康人外周血单个核细胞,并对树突状细胞进行体外定向诱导分化和培养,FACS检测DC细胞表面分子的表达。结果在加入减毒耐药变形杆菌共培养后,慢性乙肝病人CD80,CD86的表达率显著增加(P=0.000,P=0.000),而健康人的CD80、CD86表达阳性百分率改变不显著(P=0.185,P=0.118)。结论减毒耐药变形杆菌能够显著增加慢性乙肝病人外周血中树突细胞CD80和CD86阳性细胞百分率,对正常人外周血中树突细胞CD80和CD86表达的阳性细胞百分率并不增加。

Objective To explore the effect of the drug-resistant attenuated Bacillus prote us on the protein expression of CD80 and CD86 in peripheral blood dendritic cells （DCs） of hepatitis B patients. Methods Peripheral blood monocytes were isolated from HBV-infected patients and the DCs were separated and induced to differentiate in vitro. The expression of CD80 and CD86 proteins on cultured DCs were examined using flow cytometry, Results The expression rate of CD80 and CD86 of chronic hepatitis B patients increased significantly （P=0.000）, while the positive expression rate of CD80 and CD86 showed no obvious variation in healthy individuals （P=0.185 and P=-0.118, respectively）. Conclusion Bacillus proteus can significantly increase CD80- and CD86-positive cell percentage in peripheral blood DCs of HBV-infected patients, but has no such an effect in healthy individuals.