摘要
研究了胶原蛋白多肽-铬(Ⅲ)(CPCC)螯合物提高糖尿病小鼠免疫功能的机制。通过腹腔注射四氧嘧啶造小鼠糖尿病模型,观察胶原蛋白多肽-铬(Ⅲ)螯合物对小鼠免疫器官的影响,血细胞染色观察中性粒细胞,用单项琼脂扩散免疫试验测定小鼠抗体,用酶联免疫法测定小鼠外周血细胞因子IL-2、IL-6,TNF-α等指标。结果表明,胶原蛋白多肽-铬(Ⅲ)螯合物可以促进小鼠分泌的IgG抗体明显增加,增强细胞因子IL-2和TNF-α的分泌,降低IL-6的分泌。胶原蛋白多肽-铬(Ⅲ)螯合物能活化免疫细胞,恢复和改善小鼠机体的免疫功能,提高小鼠机体的免疫力,一定程度地减轻四氧嘧啶的毒性作用,降低血糖。
To study the mechanism of enhancement of alloxan-induced diabetes mouse immunity by collagen peptide-chromium（Ⅲ） complex （CPCC） , Diabetes mouse model was set up by injecting alloxan and theft neutrophile, antibody and cytokine （IL-2, IL-6 and TNF-α） of peripheral blood were detected by blood cells staining, single immunodiffusion test, and ELISA method, respectively. Results showed that Collagen peptide-chromium（Ⅲ） complex could markedly stimulate the secretion of mice IgG antibody, enhance secretion of IL-2 and TNF-α cytokine, decrease the secretion of IL-6. It was concluded that Collagen peptide-chromium（Ⅲ） complex could activate immune cell, restore and improve mouse immoral function, enhance mouse immunity, reduce the toxic effect of alloxan in certain extent, then reduce the blood glucose.
出处
《现代食品科技》
EI
CAS
2009年第4期358-361,共4页
Modern Food Science and Technology
基金
天津市应用基础及前沿技术研究计划重点项目(2009年)
