摘要
2g猪组织样品经β-葡萄糖苷酸酶酶解,0.2mol/L高氯酸沉淀蛋白,用乙酸乙酯-异丙醇(6+4,V/V)和叔丁基甲醚提取,旋转蒸发器减压蒸干,正己烷脱脂后,样液用UPLC分离,电喷雾串联四级杆质谱进行检测,本方法研究的7种β-兴奋剂(克伦特罗、莱克多巴胺、沙丁胺醇、利托君、特布他林、班布特罗和异舒普林)的线性范围为0.25～500μg/kg,线性相关系数r>0.99;0.5～10μg/kg的添加水平范围内的平均回收率为75.2%～96.7%;相对标准偏差为5.6%～13.1%;方法检出限(LOD)为0.1～0.25μg/kg。
A rapid determination method for the simultaneous analysis of severβ-agonist residues（clenbuterol, salbutamol, ractopamine, ritodrine, terbutaline, isoxsuprine, Bambuterol）in swine tissue was established. An ultra performance liquid chromatography coupled electrospray ionization quadrupole tandem mass spectrometry was applied in this method. The residues were quantified with multiple reaction monitoring （MRM） mode. The analytical method showed high precision and repeatability, with the limit, linear range and recovery being of 0.1-0.25μg/kg, 0.25- 500μg/kg and 75.2%.-96.7%, respectively, which was suitable for the determination of residues ot β-agonist in swine tissue.
出处
《现代食品科技》
EI
CAS
2009年第4期451-454,共4页
Modern Food Science and Technology
