桂枝汤对动脉粥样硬化大鼠血管活性物质的影响

The Effct of Guizhi-tang on Vascular Endothelial of Atherosclerosis Rats

目的:研究桂枝汤对长期高脂饲料喂养引起的大鼠动脉粥样硬化模型血脂和血管内皮活性因子的作用。方法:48只大鼠随机分为5组:正常组(普通饲料),n=8;模型组(高脂饲料喂养复制大鼠动脉粥样硬化模型),n=10;阳性对照组(模型组加辛伐他汀灌胃),n=10;桂枝汤低浓度组(模型组加桂枝汤10g/(kg·d)灌胃),n=10;桂枝汤高浓度组(模型组加桂枝汤20g/(kg·d)灌胃),n=10。喂养14周用光镜观察动脉斑块形成后检测各组血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),放免法测定血浆内皮素(ET)和血管紧张素Ⅱ(AngⅡ)含量。结果:桂枝汤持续治疗6周后,相对于模型组,血清TG、TC、LDL-C浓度降低(P<0.01),HDL-C浓度升高(P<0.01),ET和AngⅡ含量降低(P<0.01)。结论:桂枝汤可降低血脂和血管内皮活性因子,保护血管内皮,改善血管内皮功能。

Objective：To investigate the effct of Guizhi-tang in improving vascular endothelial function of atherosclerosis rats. Methods： 48 Sprague-Dawley（SD）rats were divided randomly into five groups which were control group, atherosclerosis group, simvastatin group, Guizhi-tang[10g/（ kg· d）] group and Guizhi-tang [- 20g/（kg·d）] group. The Total cholesterol（TC）, triglyceride（TG）, low density lipoprotein（ LDL）, high den- sity lipoprotein（HDL）, Endothelin and Ang Ⅱ were observed for each group. Results： The study showed that total cholesterol triglyceride and low density lipoprotein were decreased in Guizhi-tang group. High density lipoprotein was enhanced more significantly in Guizhi-tang group than that of atheroselerosis group（P〈0. 01）. Endothelin and Ang Ⅱ were decreased in Guizhi-tang group and simvastatin group. Coulusion： the Guizhi-tang could decrease lipid and improve vascular endothelial function.