苯妥英钠对实验性腹膜炎小鼠肺损伤的干预作用

The Intervention Function of Phenytoin Sodium on Lung Injury Following Experimental Peritonitis in Mice

目的:探讨苯妥英钠对实验性腹膜炎小鼠肺组织损伤的早期干预作用。方法:18只小鼠随机分为模型组、治疗组和对照组。模型组腹腔注射冰醋酸诱发非特异性腹膜炎,观察腹腔病理学变化,测定肺组织一氧化氮(Nitric Oxide,NO)、丙二醛(Malondialdehyde,MDA),还原型谷胱甘肽(Glutothione,GSH)的含量。治疗组诱发实验性腹膜炎后腹腔注射苯妥英钠。结果:模型组小鼠肺组织内NO、MDA含量显著升高,GSH含量显著下降。治疗组的NO和MDA含量显著低于模型组,GSH显著升高。结论:实验性腹膜炎小鼠肺组织自由基增高及高浓度NO介入了损伤过程,早期应用苯妥英钠治疗有抗自由基损伤的保护作用。

Objective： To explore the mechanism of lung injury induced by experimental peritonitis and to evaluate intervention of phenytoin sodium. Methods：18 mice were randomly divided into model group,treat- ment group and control group. Non specific peritonitis was induced by injecting ice acetate acid into mouse＇s peritoneal cavity in model group. The pathologic changes of peritoneal cavity were observed and Lung speci- mens were obtained to determine the level of NO,MDA and GSH. In treatment group, mice were subjected to injecting phenytoin sodium after ice acetate acid injection setting up. Results： Content of NO, MDA in model group was increased and content of GSH was decreased significantly compared with that of control group. The drug of phenytoin sodium could decrease NO and MDA content and increase GSH content significantly. Conclu- sion：Changes in levels of NO and MDA play an important role in lung injury resulted from experimental perito- nitis. The phenytoin sodium can be against the effect of free radicals to prevent mice from damage induced by experimental peritonitis.