慢性氟中毒对大鼠脑组织脂质过氧化水平和抗氧化能力的影响

Effect of chronic fluoride poisoning on the level of lipid peroxidation and antioxygen ability in rat brain tissue

目的研究氟在大鼠脑组织中的蓄积及其对脂质过氧化水平和抗氧化能力的影响。方法用氟化钠对大鼠进行亚慢性染毒,测定大鼠脑组织中氟浓度和脂质过氧化产物丙二醛(MDA)的含量及抗氧化酶超氧化物歧化酶(SOD)、谷胱甘肽过氧化物氧化酶(GSH-Px)的活力。结果大鼠脑组织中氟浓度与染毒剂量呈正相关,高剂量组脑组织氟浓度明显高于低剂量组和对照组(P<0.01)。脂质过氧化产物MDA含量在三组间差异有统计学意义(P<0.01),高剂量组MDA含量高于低剂量组,低剂量组高于对照组。抗氧化酶SOD和GSH-Px的活力三组间差异有统计学意义(P<0.01)。但在组间比较时发现,低剂量组与对照组间差异没有统计学意义(P>0.05)。高剂量组与低剂量组、对照组间差异有统计学意义(P<0.01)。脑组织中氟浓度与MDA含量之间呈明显正相关(r=0.731,P<0.01),而与SOD和GSH-Px的相关性不明显(r分别为0.234和0.148,P>0.05),MDA与SOD和GSH-Px呈明显负相关(r分别为-0.571和0.591,P<0.01)。结论氟可透过血脑屏障在大鼠脑内蓄积。过量的氟接触将导致机体抗氧化系统紊乱,脂质过氧化程度加剧,造成脑组织氧化性损伤。

Objective To study the accumulation of fluoride in rat brain tissue and its effect on the level of lipid peroxidation and antioxidative ability. Methods Rats were subchronically exposed to NaF, and fluoride concentration in rat brain tissue, the level of lipid peroxidation and antioxidative ability SOD, GSH-Px were measured. Results Fluoride concentration in rat brain tissue was significantly cor- related with the dosage of fluoride, and the fluoride concentration in brain tissue of high-dose group was higher than that of low-dose group （P〈 0.01）. The MDA contents had significantly difference among three groups （P 〈 0.01 ）, the MDA content of high-dose group was higher than that of low-dose group, and the latter was higher than that of the control group. The activities of antioxidase SOD and GSH-Px were significantly different among three groups （P 〈 0.01 ）. But there was no significantl difference between low-dose group and control group （P 〉0.05）. As compared with low-dose and control group, the high-dose group was significantly different from three groups （P 〈 0.01）. There was significantly posi- tive correlation between the fluoride concentration in brain tissue and the MDA content （r = 0. 731, P 〈 0.01 ）, and there were significantly negative correlation between the content of MDA and the activities of SOD and GSH-Px, respectively （ r = - 0. 571 and 0. 591 respectively, P〈 0.01）. Conclusions Fluoride may go through the blood-brain barrier and accumulate in rat brain tissue, and intensify the degree of lipid peroxidation, resulting in brain combined oxidative damage.