CD44V6、E-cadherin与非小细胞肺癌骨、脑转移关系的研究

Expression and significanc of E - cadherin and CD44V6 in the metastasis of bone and brain of NSCLC

目的探讨非小细胞肺癌（NSCLC）及癌旁组织中CD44V6和上皮钙依赖黏附蛋白（E-cadherin、E-cad）的表达与NSCLC发生骨、脑转移的关系。方法采用免疫组化Envision法检测65例NSCLC组织及30例癌旁组织中CD44V6和E-cad的表达情况，分析与NSCLC骨、脑转移的关系。结果NSCLC原发病灶组织中CD44V6阳性表达率44．6％（29／65），在癌旁组织中无表达，且与NSCLC组织分型、TNM分期及骨、脑转移呈正相关（P〈0．05），E-cad阳性表达率53．8％（35／65），显著低于癌旁组织（80％）（P〈0．05），且与NSCLC分化程度、TNM分期及骨、脑转移呈负相关（P〈0．05）。结论对NSCLC进行CD44V6及E-cad的检测，有助于预测NSCLC骨、脑转移倾向和评估NSCLC患者的预后。

Objective To explore the significance of the expression of E - cad and CD44V6 in the metastasis of bone and brain of NSCLC. Methods The expression of E - cad and CD44V6 in 65 specimens of NSCLC and 30 specimens of adjacent normal tissues were detected by Envision immunohistochemistry. Result the high expression rate of CD44V6 was 44.6% in NSCLC, and 0 in adjacent normal tissues. CD44V6 staining in NSCLC tissues was correlated to classification, bone and brain metastasis and TNM stage （ P 〈 0.05 ）. The high expression rate of E - cad was significantly lower in NSCLC than that in adjacent normal tissues（53.8% vs. 80. 0% ,P 〈 0.05）. E - cad staining in NSCLC tissues was correlated to differentiation, bone and brain metastasis and TNM stage（P 〈 0.05 ）. Conclusion Determination of CD44V6 and E - cad by immunohistchemistry might be useful in assessing tumor progression and distant metastasis and in predicting prognosis of lung cancer.