速效消脂汤治疗脂肪肝的实验研究

Experimental Study on Non-alcoholic Fatty Liver with Suxiao Xiaozhi Decoction

目的:探讨速效消脂汤对脂肪乳剂所致大鼠非酒精性脂肪肝的保护作用。方法:雄性SD大鼠40只,随机分为4组,分别为正常组,模型组,速效消脂汤组和非诺贝特组。除正常组外,使用自制脂肪乳剂对其他各组予以灌胃,每次1mL/100g体重,每日1次,于造模3周后,除正常组与模型组外,其余两组在造模的同时给予相应药物进行灌胃治疗,每日给药1次;模型组在造模同时给予等体积的生理盐水,正常组每日也给予等体积生理盐水灌胃1次,连续用药4周。于末次给药12h后眼球采血,检测血清ALT、AST活性及TG、TC含量;计算肝指数;取部分肝脏组织进行匀浆,测定肝内TG、TC含量,并观察肝组织病理学变化。结果:速效消脂汤能显著降低脂肪乳剂所致大鼠非酒精性脂肪肝血清ALT、AST活性以及TG、TC含量;对肝内TG、TC也有明显降低作用;能明显改善肝组织脂肪变,与模型组比较差异有统计学意义(P<0.05)。结论:速效消脂汤对由于脂肪乳剂所造成的非酒精性脂肪肝具有良好的保肝降脂作用,其作用机制可能与其增强自由基清除及抗脂质过氧化有关。

Objective:Experimental study on the anti-rat NASH effect of Suxiao Xiaozhi Decoction(SXD).Methods:Fouty SD rats were randomly divided into 4 groups,normal group(group A),model group(group B),group of SXD(group C) and group of fenofibrate(group D).The rats in normal group were fed with normal diet,and the rats in other groups were fed with fat emulsion.After three weeks,fatty liver model was established.Then,give group C and group D the treatment,and at the same time,continue to feed the three groups with fat emulsion.The treatment will be continued for four weeks.And at the twelve hours after the last administration of the drugs,rats in all groups would be killed.Blood samples for detecting ALT,AST activity and TG,TC contents were collected.Histopathological examination was also performed for liver tissue.Results:The SXD could significantly lower serum ALT,AST activity,and lower serum TG,TC contents.In the liver tissue,SXD could lower the TG,TC contents as well.And it could markedly ameliorate the liver damage(P<0.05).Conclusions:SXD can protect liver against NASH which could induced.Maybe,the mechanism is the compound can enhance the elimination of OFR.