摘要
目的:探讨苦参药材质量标准。方法:采用薄层色谱(TLC)法对苦参药材进行定性鉴别;采用液相色谱-质谱联用仪(LC/MSD)进行苦参药材指纹图谱的确立;采用高效液相色谱法(HPLC)测定苦参中苦参碱、氧化苦参碱、氧化槐果碱含量。结果:槐定碱呈现地区性差异,以山海关为分界线,东北地区(含内蒙古东北部)苦参药材不含槐定碱,黄河流域苦参药材含槐定碱。结论:苦参碱、氧化苦参碱、氧化槐果碱为共性成份,相对含量一致,且以氧化苦参碱、氧化槐果碱含量较高;确立了苦参药材指纹图谱;建立了苦参总碱、苦参碱、氧化苦参碱、氧化槐果碱的含量测定方法。
Objective:To investigate the quality criteria of Kushen medicinal substances.Methods:The qualitation detect of the Kushen medicinal substances has completed by using TLC.Established the finger print of the Kushen medicinal substances by LC/MSD.Determined the content of sophor carpidine,ammothamnine,N-oxysophocarpine by HPLC.Result:The investigation suggested that sophoridine present regional disparity.When using Shanhaikwan as dividing line,Kushen medicinal substances in the northeast region(including northeast part of Inner Mongolia) have not contain sophoridine,while Yellow River Valley does.We also find that sophor carpidine,ammothamnine,N-oxysophocarpine are the common ingredients and have the coincident content relatively.The content of sophor carpidine and N-oxysophocarpine are higher than others.The investigation also established the finger print of the Kushen medicinal substances and determined the assaying method of Kushen total base,sophor carpidine,ammothamnine,N-oxysophocarpine.
出处
《中医药学报》
CAS
2009年第2期40-42,共3页
Acta Chinese Medicine and Pharmacology
基金
"十五"国家重大科技专项<20种中药提取物质量标准研究>之<中药苦参提取物质量标准>
课题编号:2001BA701A36-13
关键词
苦参药材
TLC
指纹图谱
质量标准
Kuhsen medicinal substances
TLC
finger print
quality criteria