不同因素对茄子花药愈伤组织诱导的影响

Effects of Different Culture Factors on Callus Rate of Eggplant Anthers

为了提高花药愈伤组织的诱导率,进一步优化培养条件,以西安绿茄为材料,研究了不同激素、碳源、氨基酸及AgNO3对花药愈伤组织诱导的影响。结果表明:1)单一激素愈伤组织诱导率几乎均为零,生长素和细胞分裂素组合的总愈伤率(愈伤率1)均大于18.0%,花药中部愈伤率(愈伤率2)均大于4.00%;其中,以0.2 mg/L 2,4-D+1.0 mg/L NAA+0.25 mg/L KT组合表现最好。2)3种碳源中,2%蔗糖愈伤组织诱导率1为57.14%,表现最好;2%的蔗糖和6.5%的葡萄糖出愈率2均大于12%,无明显差异。3)加入氨基酸及AgNO3的出愈率1和出愈率2均高于不加任何添加物的处理;100 mg/L丝氨酸对于诱导花药中部出愈率效果最好。说明,单一激素愈伤组织诱导效果较差,生长素和细胞分裂素组合诱导效果较好,西安绿茄最适愈伤诱导培养基为MS+0.2 mg/L 2,4-D+1.0 mg/L NAA+0.25 mg/L KT+2%蔗糖+100 mg/L丝氨酸。

The anthers of Xi＇an eggplant variety were cultured under different hormones, carbon sources, amino acids and AgNO3 to study the effects on the callus rate for establishing the optimum culture condition. The results showed that： 1） the callus rate was nearly zero when a single hormone was used in the medium, total callus rate and callus rate of central anther was above 18.0% and 4.0~ when auxin and cytokinin was used in the medium simultaneously and the optimum medium was 0. 2 mg/L 2,4-D＋1.0 mg/L NAA＋0. 25 mg/L KT. 2） totall callus rate was 57. 14% when 2% sucrose was used in the medium and the callus rate of central anther all was above 12 % when 2% sucrose and 6.5% glucose was used in the medium seperatly. 3）the total callus rate and callus rate of central anther of treatments with adding amino acids and AgNO3 was higher than CK. The optimum medium for improving anther callus rate of Xi＇an eggplant variety was MS＋ 0.2 mg/L 2,4-D＋ 1.0 mg/L NAA＋0.25 mg/L KT＋2% sucrose ,4-100 mg/L Ser.