丙酮酸钙对小鼠力竭运动能力的研究

Effects of calcium pyruvate supplementation on exercise ability in mice

[目的]探讨补充3周丙酮酸钙对于小鼠力竭运动能力的影响。[方法]雄性小鼠40只,随机分为4组,分别为安静对照组、运动对照组、丙酮酸钙组、运动丙酮酸钙组。给药组每天灌胃给予600 mg/(kg.d)的丙酮酸钙共3周。3周末进行力竭运动实验,测各组运动至力竭时间,并取右侧腓肠肌组织和肝脏组织分别测肌糖原、肝糖原。[结果]与运动对照组(106.85±24.66)min比较,运动给药组(166.95±31.18)min鼠运动至力竭时间明显延长,运动能力提高,差异有显著性意义(P<0.001)。运动前丙酮酸钙组肌糖原、肝糖原含量、超氧化物歧化酶(SOD)含量明显高于安静对照组,差异有显著性意义(P<0.05)。运动力竭后各组肌糖原、肝糖原含量没有明显差异。与运动对照组比较,运动给药组SOD明显升高,差异有显著性意义(P<0.05)。运动对照组肝脏丙二醛(MDA)的含量均高于其他三组,差异有显著性意义(P<0.01);运动给药组MDA含量明显低于运动对照组,差异有非常显著性意义(P<0.001)。血清钙浓度各组间差异无显著性意义(P>0.05)。[结论]补充3周丙酮酸钙能明显提高小鼠力竭运动能力,其可能的原因是提高运动前肌糖原和肝糖原水平。

[ Objective] To investigate the effects of calcium pyruvate on exercise ability in mice for 3 weeks. [ Methods ] Forty mice were randomly divided into four groups： control group, exercise control group, pyruvate group and exercise pyruvate group . The mice in pyruvate groups received calcium pyruvate and the mice in control groups received placebo 600 mg per weight per day for 3 weeks. Exercise ability and glycogen were measured after the experiment. [ Results ] At the end of the experiment, there was a statistically significant improvement of exercise ability in exercise pyruvate group and an increase of the liver and muscle glycogen and SOD in pyruvate supplementation group . There was no significant difference in the liver and muscle glycogen between exercise control group and exercise pyruvate group at the end of exhaustive exercise. There was a statistically significant increase in SOD and a decrease in MDA between exercise control group and exercise pyruvate group at the end of exhaustive exercise. [ Conclusion ] The results suggest that supplement of calcium pyruvate for 3 weeks can improve exercise ability for mice.