镉离子胁迫下4种辣椒超氧化物歧化酶同工酶酶谱及酶活性分析

Catalytic Activity and Isozyme Patterns of Superoxide Dismutase in Four Species of Capsicums under Cd^(2+) Stress

以4种辣椒为材料,分析在不同胁迫浓度和胁迫时间镉离子胁迫下,4种辣椒超氧化物歧化酶(SOD)活性及同工酶酶谱的变化。结果表明,镉离子能显著影响酶活性的变化,不同镉离子浓度胁迫下不同材料酶活性变化不同, SOD酶活性最高为对照组的1.4倍(1号品种,100 mg/L胁迫3 d),酶活性最低的为对照组的60%(2号品种,150 mg/L胁迫6 d);镉离子还能影响同工酶酶谱,不同胁迫时间和浓度对同工酶的影响不同,对1号材料的影响最为显著,能诱导出2条SOD同工酶新谱带。

Catalytic activity and isozyme patterns of superoxide dismutase （SOD） were studied in four species of capsicums at different times under series concentration of cadmium （Cd）. The results show that Cd^2＋ can affect catalytic activity of SOD remarkably and dissimilarly according to its concentration. The highest catalytic activity is 1.4 times higher compared to the control （No. 1 species, 100 mg/L for 3 d） while the lowest is only 60% of that of control （No. 2 species, 150 mg/L for 6 d）. Besides, Cd^2＋ can also affect isozyme patterns of SOD, especially in No. 1 species. Two new isozyme bands are induced.