甲氨蝶呤对银屑病CXCR3mRNA表达调控的研究

Inhibition effects of methotrexate on CXCR3 mRNA expression in patient with psoriasis vulgaris

目的研究甲氨蝶呤(MTX)对银屑病患者皮损内趋化性细胞因子受体CXCR3表达的影响。方法利用荧光定量聚合酶链反应法测定20例寻常型银屑病患者治疗前后皮损内及17例正常对照者皮肤中CXCR3mRNA的定量表达,以直线相关回归分析CXCR3mRNA的表达与银屑病病情严重程度(PASI)的关系。结果银屑病皮损内CXCR3mRNA高于正常对照者皮肤内的表达水平(P<0.01)。银屑病皮损内CXCR3mRNA的表达与患者病情严重程度呈显著的正相关(r=0.72465,P<0.01)。银屑病患者进过MTX治疗6周后,皮损明显改善,皮损内CXCR3mRNA的表达水平低于银屑病患者治疗前皮损内的表达水平(P=0.0003),但与正常对照者皮肤内的表达水平无统计学差异(P=0.0756)。结论趋化性细胞因子受体CXCR3可能在银屑病的发病中其重要作用。MTX能抑制银屑病皮损内CXCR3mRNA的过度表达,这可能是MTX治疗银屑病的作用机制之一。

Objective To determine the effect of methotrexate on the expression of chemokine receptor CXCR3 in psoriatic lesional skin. Methods Twenty adult patients with psoriasis vulgaris and 17 health controls were sequentially enrolled in this study. The production of CXCR3 mRNA was measured by real-time PCR. The correlation of level of CXCR3 with the severity of the disease was assessed. Results Expression of CXCR3 mRNA in psoriasis lesional skin was higher than that of the controls （P〈0.01）. Expression of CXCR3 mRNA was markedly positively correlated with severity of the disease （r=0.72465,P〈0.01）. Expression of CXCR3 mRNA in psoriasis lesional skin was decreased 6 weeks after methotrexate treatment （P=0.0003）. Expression of CXCR3 mRNA from psoriatic resolving lesions after treatment similar to the controls （P=0.0756）. Conclusions It show that CXCR3 play an important role in the development and advance of psoriasis vulgaris. MTX could inhabit the over expression of CXCR3 mRNA. These changes might be part of the mechanism of MTX on psoriasis.