野生欧洲李组织培养技术

Tissue Culture Techniques of Prunus domestica L.

以濒危果树野生欧洲李当年生新梢为外植体，进行组织培养研究，以期筛选出最优培养基配方及培养程序。结果表明：野生欧洲李初代培养时，以B5为基本培养基优于MS培养基；培养基中添加一定量激素的外植体生长势明显优于不添加激素；以B5为基本培养基，6-BA质量浓度0．5～1．0mg／L，并添加一定量的生长素（IBA、NAA）效果较优，能较快启动生长反应，并初步形成一定的增殖率；芽增殖培养阶段，6-BA质量浓度为0．5～1．0mg／L时，以NAA为生长素，NAA和6-BA适宜的比例为1：（1～3），以IBA为生长素，IBA和6-BA适宜的比例为1；（3～5）较适宜。

In order to select out optimum media formula and culture program, tissue culture techniques of wild European plum （Prunus dornestica L. ） were researched, which belong to endangered fruit tree. The results showed that Bsbasic medium was better than MS medium in initial culture; Explants had better growth vigor in the media supplemented with some hormones than that in the media without any hormone ; Explants started to grow more quickly in Bs supplemented with 6-BA 0. 5-1.0 mg/L and growth hormone （NAA or IBA）, and some proliferation buds could be gained; The suitable concentration ratio of NAA to 6-BA was 1 ： （1--3） in the media supplemented with 6-BA 0.5--1.0 mg/L and NAA for proliferation of buds, and that of IBA to 6-BA was （1 ： 3--5） in the media supplemented with 6-BA 0. 5-1.0 mg/L and IBA.