不同温度和pH值对瘤背石磺(Onchidium verruculatum Cuvier)消化酶活性的影响

Effect of Different Temperatures and pHs on Digestive Enzyme Activities of Onchidium Verruculatum Cuvier

研究了温度和pH值对瘤背石磺(Onchidium verruculatum Cuvier)肠淀粉酶、肝胰腺淀粉酶和胃淀粉酶以及相应的纤维素酶活性的影响.不同温度和pH值对瘤背石磺消化酶活性的影响显著(P<0.05).当温度在5–75℃时,肠淀粉酶和纤维素酶分别在35℃和30℃时活性较高;肝胰腺淀粉酶和纤维素酶分别在35–45℃和50℃时活性较高;胃淀粉酶活性在25–45℃维持较高水平,随后随温度的升高而下降,纤维素酶活性在50℃时较高.在pH为3.0–8.0范围内,肠淀粉酶和纤维素酶的最适pH值均为6.0;肝胰腺淀粉酶和纤维素酶的最适pH值分别为6.9和5.4;胃淀粉酶和纤维素酶的最适pH值分别为6.5和6.0.在适宜温度和pH条件下,肠淀粉酶、肝胰腺淀粉酶和胃淀粉酶活性均远大于肠纤维素酶、肝胰腺纤维素酶和胃纤维素酶.

The effects of different temperatures and pHs on activities of intestines amylase, hepatopancreas amylase and stomach amylase as well as intestines cellulose, hepatopancreas cellulose and stomach cellulose of Onchidium verruculatum Cuvier were studied in this paper. The results showed： different temperatures and pHs have affected the activities of amylase and cellulose of Onchidium verruculatum Cuvier significantly （P 〈 0.05）. Between the temperature 5℃and 75℃, intestines amylase and cellulose are highly active at the temperature of 35℃ and 30℃, while the hepatopancreas amylase keeps a high activity at the range of 35 - 45 ℃ and the cellulose at 50℃. And the stomach amylase has temperature optima range at 25 - 45 ℃ and the cellulose has temperature optima at 50℃. Between the pHs 3.0 and 8.0, the optima pHs value of the intestines amylase and cellulose are 6.0; while the optima pHs value of the hepatopancreas amylase and cellulose are 6.9 and 5.4; and the stomach amylase and cellulose are 6.5 and 6.0. The result also showed that at the most favorable reacting temperature and pH of the two digestive enlzymes, the activities of the amylase are much higher than those of the cellulose.