钙稳态失衡在氯化镧诱导MDCC-MSB1细胞凋亡中的作用(英文)

Effect of disequilibrium of calcium homeostasis on apoptosis of MDCC-MSB1 cells induced by lanthanum chloride

为探讨钙稳态失衡在LaCl3诱导MDCC-MSB1细胞凋亡中的作用,MDCC-MSB1细胞常规培养于RPMI1640培养液中,加入终浓度为0.5,1,1.5,2,2.5,3,3.5和4mmol·L-1的LaCl3,继续培养24h后,应用MTT法检测细胞增殖抑制率,DNA Ladder法和TUNEL法检测细胞凋亡,以Fura-2为荧光探针检测细胞内[Ca2+]i的变化。结果表明,在LaCl3浓度为0.5～4mmol·L-1时,细胞的增殖抑制率增加,细胞凋亡数量和细胞内[Ca2+]i呈升高趋势,并呈剂量-效应关系。这表明LaCl3能抑制MDCC-MSB1细胞的增殖,并可能通过改变[Ca2+]i而诱导其发生凋亡。

To investigate the effect of the disequilibrium of calcium homeostasis on the apoptosis of MDCC-MSB1 cells induced by lanthanum chloride （LaCl3）, MDCC-MSB1 cells was conventionally cultured in RPMI1640, which was added with differently final concentration of LaCl3（0. 5,1,1.5,2,2.5,3,3.5 and 4 mmol·L-1 ）. After being cultured for 24 h,MTT was utilized to detect the cell muhiplication inhibition ratio,DNA ladder and TUNEL to detect apoptosis,the Fura-2/AM as the probe to detect the [Ca^2＋ ]i within cells. The results indicated when LaCl3 concentration varied from 0.5 mmol·L-1 to 4 mmol·L-1 , the cell multiplication inhibition ratio increased, the apoptosis and [Ca^2＋]i also increased,and presented a dose-effectiveness relationship. LaC13 could restrain the proliferation of MDCC-MSB1 cells,suggesting their apoptosis induced by changing [Ca2＋]i.