高效液相色谱法测定牛黄上清片中栀子苷的含量

Content determination of geniposide in Niuhuang Shangqing Tablets by HPLC

目的:建立牛黄上清片中栀子苷的含量测定方法。方法:采用高效液相色谱法测定牛黄上清片中栀子苷的含量。色谱柱:Dikma Kromasil C18柱(250×4.6mm,5μm);流动相:乙腈-水(15:85);检测波长:238nm;柱温:25℃;流速:1.0ml/min;进样量:10.0μl。结果:栀子苷在0.4204～4.204μg范围内有良好的线性关系(r=0.9998),平均回收率为98.03%,相对标准差(RSD)为1.15%(n=6)。测得牛黄上清片中栀子苷的平均含量为2.3848mg/g。结论:该方法简便易行、快速准确,具有良好的重复性和回收率,可作为牛黄上清片中栀子苷的定量分析方法。

Objective ：To establish a method for the content determination of geniposide in Niuhuang Shangqing Tablets.Methods： The content of geniposide was detected by HPLC.The separation was carried out on a Dikma Kromasil C18 column （5 μm,250×4.6 mm）. The mobile phase was acetontrile-water（15：85）;the flow rate was 1.0ml/min ;the detective wavelength was set at 238 nm;the column temperature was 25℃ and the sample size was 10.0 μl.Results：The calibration curve showed good linearity over the range of 0.4204-4.204 μg （r=0.9998）.The average recovery rate was 98.03% with RSD 1.15%（n=6）.The sample contained geniposide 2.3848 mg/g. Conclusion： This method was simple,rapid,accurate with good repeatability and recovery,it can be used as a quantitative analysis method for the content determination of geniposide in Niuhuang Shangqing Tablets.