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强的松对CⅡTA调节HLA作用的影响

The regulation of prednisone on expression of CⅡCA for accommodation of HLA in activated PBMCs
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摘要 目的:研究强的松对人PBMCCⅡTA转录的调节,从基因水平探讨糖皮质激素对HLA的调控作用及其调控机制。方法:用强的松干预经IFN-γ联合PHA刺激诱导活化的正常人PBMC,采用RT-PCR方法扩增其CⅡTA mRNA,并对处理后PBMCCⅡTA转录水平与未诱导的组成性及活化后的诱导性CⅡTA转录水平进行分析比较。结果:体外培养的正常人PBMC组成性表达CⅡTA,经IFN-γ联合PHA活化后表达增加,强的松干预后,表达量明显减少。结论:强的松对IFN-γ诱导活化的PBMCCⅡTA转录有抑制作用,对HLA表达的抑制作用可能与下调CⅡTA转录有关。 Objective： CⅡTA is a transcriptional factor for transaetivation of HLA expression. The aim of the study is to investigate the regulation of prednisone on inducible expression of CⅡTA in native PBMCs.Methods： Normal PBMC was stimulated with combination of IFN- γ and PHA with or without the presense of prednisone. CⅡTA mRNA in the cells was amplified by RT-PCR, for analyzing transcriptive level in three designed groups of native PBMCs, activated PBMCs using the biomodulators and the triggered PBMCs in presense of prednisoloen. Results：Normal human PBMCs constitutively expressed CⅡTA, which was increased by stimulation of PHA and IFN-γ. Prednisone significantly inhibited the inducible pattern of CⅡTA expression. Conclusion： Prednisone has showed inhibition effect on the expression of PBMC CⅡTA by descending transcription of the specialized transcriptional factor in activated PBMCs, whereby to downregulate the expression of HLA in PBMCs.
作者 王桂琴 李凌霞 兰涛
机构地区 山西医科大学基础学院微免教研室 山西财经大学医院 山西省人民医院儿科
出处 《中国免疫学杂志》 CAS CSCD 北大核心 2009年第4期295-298,共4页 Chinese Journal of Immunology
基金 山西省科技攻关项目基金资助课题(No.051097-1)
关键词 HLA CⅡTA 强的松 免疫抑制 HLA CⅡTA Prednisone Immune-suppression
分类号 R657.3 [医药卫生—外科学] R363.27 [医药卫生—病理学]
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参考文献8

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二级参考文献12

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中国免疫学杂志
2009年 第4期

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