泥胡菜中芦丁含量分析

Analysis of Content of Rutin in Hemisteptia lyrata Bunge

建立高效液相色谱法测定泥胡菜中芦丁的方法，优化芦丁的提取工艺。使用Diamonsil（TM）C18柱，流动相：乙腈-0．4％磷酸溶液（20：80），检测波长：255nm，流速：1ml／min，柱温：30℃。样品用50％乙醇超声提取30min提取率最高为0．166％；芦丁在0．1～2．0μg范围内与峰面积呈良好的线性关系（r=0．9994），平均加样回收率99．3％（RSD=0．8％，n=5）。该方法准确、快速、重现性好，可作为控制泥胡菜质量的有效测定方法。

To establish an HPLC method for determination the content of Rutin in Hemisteptia lyrata Bunge. The Diamonsil^（TM） diamond C18 column （250 mm ×4.6 mm, 5 μm） was used. The mobile phase consisted of Acetonitrile 0.4% H3PO4 solution （20： 80）, the detection wave length was 255 nm. Flow rate was lmL/min and column temperature at 30 ℃. The maximum extraction rate is 0. 166% with the extraction solvent is 50% alcohol and the extraction time is 30 minutes ; the linear range of Rutin was 0. 1 -2.0μg （r =0. 999 4）, the mean recovery of Rutin was 99.3% （RSD =0.8%, n =5）. This method is accurate, rapid and reproducible, can be used to determine rutin in the material of Hemisteptia lyrata Bunge.