泽泻的化学成分及其免疫抑制活性筛选

Chemical constituents of Alisma orientalis and their immunosuppressive function

目的:研究泽泻Alisma orientalis的免疫抑制活性物质。方法:利用色谱技术进行分离和纯化,并通过现代波谱方法鉴定其结构,采用体外细胞培养法,测定萜类化合物对小鼠脾脏T淋巴细胞增殖的活性。结果:从泽泻中分离鉴定了12个化合物。分别为倍半萜类clovandiol(1),orientalol E(2),alismoxide(3),alismol(4)和三萜类4α,10α-dihydroxy-5β-H-guaj-6-en(5),alismorientols A(6),泽泻醇F(7),泽泻醇A(8),13β,17β-环氧泽泻醇A(9),23-乙酰泽泻醇B(10),1H-indole-3-carboxylic acid(11)和琥珀酸(12)。化合物9,10和24-去乙酰泽泻醇A均具显示了剂量依赖性免疫抑制趋势。结论:化合物1,5,11和12均为首次从该属植物中分离得到,首次对化合物1的NMR波谱数据归属问题进行了修正,活性结果显示原萜烷型三萜类化合物具有一定的免疫抑制活性。

Objective： To investigate the chemical constituents with immunosuppressive function from Alisma orientalis. Method： The chemical constituents were isolated and purified by kinds of column chromatography and its structures were elucidated by NMR spectra and physicochemical properties. Its immunocompentence of lymphocytes taken from spleen of mouse were examined by MTT assay. Result： Twelve compounds were isolated and identified as clovandiol （1）, orientalol E （2）, alismoxide （3） , alismol （4） , 4α, 10a-dihydroxy-5β-H-guaj-6-en （5）, alismorientols A （6）, alisol F （7）, alisol A （8）, 13β,17β-epoxy alisol A （9）, alisol B 23-ace- tate （10） , 1H-indole-3-carboxylic acid （11） and cuecinic acid （12）. Compounds 9, 10 and alisol A 24-acetate showed immunosup- pressive function. Conclusion： Compounds 1, 5, 11 and 12 were isolated firstly from this genus,and the NMR spectra data of 1 were corrected firstly, some protostan-type triterpenoids may be developed as new drug with immunosuppressive function.