芪参益气方对急性心肌梗死大鼠心肌蛋白质组的影响

Analysis of myocardial proteomic alteration after Qishenyiqi formula treatment in acute infarcted rat hearts

目的:应用蛋白质组学技术研究芪参益气方对急性心梗大鼠心肌蛋白质组表达的影响,在蛋白质水平上探讨芪参益气方心肌保护的作用机制。方法:收集假手术、急性心肌梗死以及芪参益气方给药大鼠的心脏,使用双向电泳和基质辅助激光解吸离子质谱,分析比较各组大鼠心肌蛋白质组表达的差异。结果:与心肌梗死模型组相比,芪参益气方给药组心肌组织中有9个蛋白点的表达量显著上调,5个蛋白点的表达量显著下调。这些蛋白点的功能与能量代谢、氧化应激及细胞骨架构成等相关。结论:芪参益气方能够部分回复心肌损伤过程中的能量供应,减少氧化应激,促进受损细胞恢复,从而达到其心肌保护作用。

Objective： Use proteomic technique to research the effects of Qishenyiqi formula in acute myocardial infarcted rats, and further explore its protein-level mechanism. Method： The rat hearts of sham, myocardial infarcted and Qishenyiqi formula treated groups were collected. Analyzing and comparing the differences of protein expressions in each groups with two-dimensional electropho- resis, computer-assisted image analysis and matrix-assisted laser desorption/ionization mass spectrometry. Result： Compared with myocardial infarcted group, there were 14 protein expressions changed in Qishenyiqi formula treated group. The result showed that 9 protein expressions were up-regulated, and 5 ones were down-regulated significantly. These proteins are associated with energy metabolism, mitochondrial function, oxidative stress, cytoskeleton and others. Conclusion： Myocardial protective effects of Qishenyiqi formula in myocardial infarcted procedure might be closely related to the recovery of energy supply, the reduction of oxidative stress, as well as the promotion of cell recovery.