纳米氧化铝对神经小胶质细胞N9的细胞毒性初探

Toxicity of Nano Aluminum Oxide to Cultured N9 Cells

目的研究纳米氧化铝颗粒(NAOs)对神经小胶质(N9)细胞的体外毒性作用。方法观察0～125μg/ml NAOs染毒24h后N9细胞形态的改变,采用MTT方法检测0～500μg/ml NAOs和非纳米氧化铝颗粒(nNAOs)对细胞活性的影响,Hoechst33258染色观察0～500μg/mlNAOs和nNAOs对细胞凋亡的影响,流式细胞术检测0～200μg/ml NAOs对细胞凋亡率的影响。结果体外培养的N9细胞经NAOs染毒后,形态学发生明显改变。NAOs可引起N9细胞发生时间、剂量依赖性的细胞凋亡。与空白对照组相比,8、100和200μg/ml NAOs组的细胞凋亡率均较高,差异有统计学意义(P<0.05)。结论NAOs在体外实验中对N9细胞具有促凋亡作用。

Objective To determine the toxice effects of nano aluminium oxide （NAOs） to N9 cells in vitro. Methods The morphological changes of N9 cells induced by 0-125 μg/ml NAOs and nNAOs were observed with phase-control microscope. The viability and apoptosis of N9 cells line after being exposed to 0-500 μg/ml NAOs and nNAOs 24 h were detected by MTr and Hoechst 33258. Flow cytometry was used to quantify the efficiency of N9 apoptosis induced by 0-200μg/ml NAOs. Results After being exposused to NAOs,the morphology of N9 cell changed,and cell apoptosis was observed in a time- and dose-depended manner. The efficiencies of apoptosis induced by 8,100 and 200μg/ml aluminium oxide nanoparticles significantly increased compared with control （P〈0.05）. Conclusion NAOs may induce apoptosis of N9 cell line in vitro.