摘要
目的:用高效液相色谱法测定鼻炎糖浆中黄芩苷的含量。方法:采用Diamonsil C18色谱柱,以甲醇-0.4%磷酸(48:52)为流动相.流速1.0mL/min,检测波长为280nm。结果;黄芩苷在49.78~398.2μg/mL范围内呈良好的线性关系(r=0.9997),平均回收率为99.14%,RSD为0.88%(n=9)。结论:本方法简便、准确、重现性好,可有效控制鼻炎糖浆的质量。
Objective:To establish a method for the quantitative determination of Baicalin in Biyan Syrup by HPLC. Methods:We used Diamonsil C18 column, used Methanol--0. 4%Phosoporic acid solution(48: 52) as mobile phase, used lmL.min-1 as the flow rate and used 280nm as detection wavelength. Results:The results showed a good linearity in the range of 49.78-398.2μg/mL( r = 0. 9997) . The recovery was 99. 14% and RSD was 0.88 %. Conclusion : The method is sensitive, simple, dependable and accurate, which, therefore, can be used to control the quality of Biyan Syrup.
出处
《黑龙江医药科学》
2009年第2期22-22,共1页
Heilongjiang Medicine and Pharmacy