培养条件下酵母菌吸附铀的研究

Research on the Yeast Biosorption of Urantum Under Culture Conditions

研究在培养条件下对活酵母菌吸附铀的能力作了初步研究。利用偶氮胂Ⅲ作为显色剂采用分光光度法测定铀浓度的变化,结合标准曲线计算生长曲线,对酵母生长的抑制及吸附率。结果表明:酵母菌生长曲线基本符合S型生长曲线模型;低铀浓度、短培养时间时铀对酵母菌生长起促进作用,当铀浓度达到0.6mg/mL时才明显表现为抑制作用;铀浓度对吸附率的影响曲线符合典型的吸附等温线模型,随着铀浓度增大,吸附量总趋势增大;在培养条件下酵母菌对铀的处理以6～10h为佳;在低铀浓度下,铀的吸附可由Langmuir和Freundlich吸附模型模拟。

The biosorption capacities of uranium by yeast （Saccaromyces cerevisiae） under culture condition were examined. The uranium determined by spectrophotometry with Arsenazo Ⅲ .The growth curve, adsorption ratio and adsorption capacity were calculated with standard curve. Results showed that the growth curve 5f the yeast primary met the S-growth curve model; the repressive effect curve indicated that low concentration uranium and short time culture can promote the growth of the yeast, the obvious inhibition of yeast growth can be found when uranium concentration reaching 0.6mg/mL; the uranium concentration - adsorption rate curve met the isothermal adsorption curve model, the uranium concentration - the adsorption capacity curve demonstrated the total adsorption capacity was increased with the addition of the uranium concentration; the curve of the culture time-the adsorption rate suggested that the best culture time for the yeast adsorbing the uranium was 6-8 hours. The model of biosorption has been proved in agreement with Langmuir and Freundlich model by connection of uranium equilibrium concentration and adsorption capacity under low uranium concentration.