牙髓卟啉单胞菌内毒素对成骨细胞表达IL-1β mRNA和IL-6 mRNA的影响

Effects of lipopolysaccharides extracted from Porphyromonas endodontalis on the expression of IL-1β mRNA and IL-6 mRNA in osteoblasts

目的:研究牙髓卟啉单胞菌(P.e)内毒素(LPS)对成骨细胞表达炎症因子白介素-1β(IL-1β)和白介素-6(IL-6)的影响,探讨P.e-LPS参与根尖周病变牙槽骨吸收的病理机制。方法:应用反转录-聚合酶链式反应(RT-PCR),检测在不同浓度P.e-LPS(0～50μg/mL)和不同作用时间(0～24h),P.e-LPS诱导成骨细胞表达IL-1β mRNA和IL-6 mRNA的水平。应用SPSS11.0软件包对结果进行单因素方差分析和Dunnettt检验。结果:当P.e-LPS浓度大于5μg/mL,作用1h后,与0μg/mL和0h组比较,IL-1β mRNA和IL-6 mRNA的表达水平均显著提高(P<0.01),表明P.e-LPS诱导成骨细胞表达IL-1β mRNA和IL-6 mRNA具有剂量和时间依赖性。结论:P.e-LPS诱导成骨细胞表达IL-1β mRNA和IL-6 mRNA,是加速根尖病变的重要毒力因子。

PURPOSE： To quantify the IL-1β mRNA and IL-6 mRNA expression induced by lipopolysaccharides （LPS） extracted from Porphyromonas endodontalis （P.e） in osteoblasts, and to relate P.e-LPS to bone absorption pathogenesis in lesions of chronical apical periodontitis. METHODS： MG63 was treated with different concentrations of P.e-LPS（0-5μg/ mL） for different hours （0-24h）. The expression of IL-1β mRNA and IL-6 mRNA was detected by reverse transcription polymerase chain reaction （RT-PCR）.Statistieal analysis was performed using one- way ANOVA and Dunnett t test with SPSS11.0 software package. RESULTS： The level of IL-1β mRNA and IL-6 mRNA increased significantly after treatment with P.e-LPS at more than 5μg/mL（P〈0.01）and for more than 1 hour（P〈0.01） ,which indicated that P.e-LPS induced osteoblasts to express IL-1β mRNA and IL-6 mRNA in dose and time dependent manners. CONCLUSION： P. e-LPS may promote bone resorption in lesions of chronieal apical periodontitis by inducing IL-1β mRNA and IL-6 mRNA expression in osteoblasts.Supported by College Scientific Research Plan of Liaoning Province （Grant No. 2008819）.