黏性放线菌和牙龈卟啉单胞菌间共聚对黏性放线菌黏附于S-HA的影响

The effect of coaggregation of A.viscosus and P.gingivalis on the adherence of A.viscosus to hydroxyapatite

目的:研究黏性放线菌(A.viscosus)和牙龈卟啉单胞菌(P.gingivalis)间共聚对A.viscosus黏附于唾液包被的羟基磷灰石(S-HA)的影响。方法:将0.5mL5×108CFU/mLA.viscosus分别与0.5mL1×109、3×109、5×109CFU/mL的P.gingivalis菌液混合,室温旋转,SEM观察P.gingivalis与A.viscosus间的共聚;将不同浓度的P.gingivalis和用放射性核素标记的A.viscosus混合,室温旋转1.5h,荧光闪烁记数法检测A.viscosus对S-HA的黏附量,以单菌种的A.viscosus作为对照,设定其黏附量为100%,采用SPSS10.07软件包对数据进行单因素方差分析,比较不同浓度P.gingivalis对A.viscosus黏附的影响。结果:1个A.viscosus的细胞表面可黏附多个P.gingivalis。P.gingivalis浓度较小时,A.viscosus对S-HA黏附量相对于对照组略增加;随P.gingivalis浓度增加,A.viscosus黏附量显著下降(P<0.05)。结论:A.viscosus和P.gingivalis可发生细菌之间的共集,这种共集可影响A.viscosus对S-HA的黏附。

PURPOSE： To examine the effect of coaggregation of A.viscosus and P.gingivalis on the adherence of A. viscosus to saliva-coated hydroxyapatite（S-HA）. METHODS： The suspensions of A.viscosus and P.gingivalis were rotated and dropped on cover slips to visualize the coaggregation under scanning electron microscope （SEM）. Different concentrations of P.gingivalis were mixed with A.viscosus radiolabeled with 10 uCi of [methyl-^3H] thymedine per milliliter, then rotated for periods up to 1.5 hours at room temperature and the numbers of absorbed A.viscosus cells were determined by liquid scintillation. A.viscosus alone was used as the control, and its adhesion percent was set for 100%. One-way ANOVA with SPSS10.07 software package was used to analyze the effect of P.gingivalis on adherence of A. viscosus. RESULTS： Several cells of P.gingivalis could aggregate on the surface of A.viscosus. The adherence of A. viscosus to S-HA was promoted slightly when the concentration of P.gingivalis was rather small, then further increase in concentration of P.gingivalis resulted in a steady decline in the number of adherent A.viscosus, and the difference was significant （P〈0.05）. CONCLUSION： These resuhs show that the adherence of A.viscosus to SHA could be influenced by coaggregation of P.gingivalis and A.viseosus.