柿叶黄酮对大鼠急性前脑缺血再灌注损伤的保护作用

Neuroprotective Effect of Flavonoid from the Leaves of Diospyros Kaki on Acute Forebrain Ischemia Reperfusion Injury in Rats

目的:研究柿叶黄酮对大鼠急性前脑缺血再灌注损伤的保护作用。方法:采用大鼠Pulsinelli四血管阻断方法造成前脑缺血再灌注损伤模型,予柿叶黄酮不同剂量灌胃给药12天,每天2次,实验大鼠分别在造模后第8天处死,取大鼠脑海马组织制作石蜡切片,光镜观察脑海马神经细胞及组织形态学变化。结果:柿叶黄酮高、中、低剂量均能提高大鼠急性前脑缺血再灌注损伤后活CA1锥体神经细胞密度,与模型组比较有显著性差异(P<0.01)。结论:柿叶黄酮对大鼠急性前脑缺血再灌注损伤的神经元具有保护作用。

Object：To investigate the neuroprotective effect of Flavonoid from the leaves of Diospyros Kaki （FLDK） on acute forebrain ischemia reperfusion injury in rats. Methods： The forebrain ischemia reperfusion injury model was produced by Pulsinelli＇s fourvessel occlusion in rats, different dosages of Flavonoid from the leaves of Diospyros Kaki were intragastric administrated, twice a day for 12 days in total, the rats were separately sacrificed on the eighth day after modeling, and the rats＇ cerebral hippocampus were taken out for paraffin processing, the cerebral hippocampus neurons and histological changes were observed by light microscope. Results： Flavonoid from the leaves of Diospyros Kaki with high, medium and low dosage could improve the active CAlvertebral neurons density after acute forebrain ischemia reperfusion injury in rats, with a significant difference in comparison with those in the model group （P〈0.01）. Conclusion： Flavonoid from the leaves of Diospyros Kaki has the neuroprotective effect on acute forebrain ischemia reperfusion injury in rats.