能量可控陡脉冲对人卵巢癌细胞细胞骨架作用的实验研究

Experimental Study on Effects of Energy Controllable Steep Pulses on Cytoskeleton of Human Ovarian Cancer Cells SKOV3

探讨能量可控陡脉冲(Energy controllable steep pulses,ECSP)对人卵巢癌细胞SKOV3(Human ovarian cancer cells,SKOV3)细胞骨架的影响。将SKOV3分成5组,A组用假电击处理作为阴性对照组,B、C、D组用不同参数的能量可控陡脉冲处理,E组用秋水仙碱处理的SKOV3细胞作为阳性对照组。以罗丹明-鬼笔环肽直接标记微丝,以免疫荧光法标记微管,用激光共聚焦显微镜观察。制作电镜标本,用电子显微镜观察细胞骨架的超微结构。结果显示能量可控陡脉冲作用的SKOV3细胞微丝明显解聚、骨架蛋白弥散分布、细胞骨架的网络结构消失;微管解聚,伪足减少且其中的微丝明显解聚,排列紊乱,结构消失。而且这一作用与剂量呈正相关。

The aim of our study was to determine the effects of energy controllable steep pulse（ECSP） on the eytoskeleton of human ovarian cancer ceils SKOV3. SKOV3 ceils were divided into five groups under ECSP treatment with different parameters（frequency,pulse duration,peak value of voltage）. The positive control group included SKOV3 cells treated with volchicine; the negative control group included SKOV3 cells subjected to sham-lightning stroke. Rhodamine-phalloidine was used to label mierofilament directly. After using immunofluorescence to label microbules, we observed them by means of Confocal Laser Scanning Microscope. Making specimen and using electron- microscope, we observed the ultramicrostructure of cystoskeleton. The results showed that ECSP-treated-SKOV3 cells lost their normal cystoskeleton network structure. There were obvious microfilament disaggregation, diffused skeleton protein, and disappearance of cystoskeleton network structure. Also noticeable were mierobule disaggregation, reduction of pseudopod, obvious microfilament disaggregation, permutation disorder and structur disappearanee. Moreover, this effect bears a direct relation with dosage.