染料介导光氧化法处理的同种异体动脉体内近期生物稳定性和细胞毒性研究

Research on Short-term Biological Stability and Cytotoxicity of Allogenic Arteries Cross-linked by Dye-mediated Photooxidation In Vivo

观察染料介导光氧化(DMP)法处理的家兔胸主动脉用于同种异体腹主动脉置换后,在体内血流环境下的生物稳定性和细胞毒性。以经染料介导的光氧化反应处理的家兔胸主动脉为研究对象,以戊二醛(GA)处理的家兔胸主动脉以及自体腹主动脉原位移植作为对照,建立家兔胸-腹主动脉移植模型;实验动物观察饲养1个月后,取出标本观察,通过光学显微镜半定量计数观察移植血管的体内生物稳定性;通过扫描电镜半定量计数观察移植血管内皮覆盖情况,以此评价移植血管的细胞毒性。结果显示:DMP组生物稳定性指数较自体动脉移植组低,差异不具统计学意义(P>0.05),DMP组生物稳定性指数较GA组高,差异具显著统计学意义(P<0.01);DMP组内皮覆盖率较GA组高,差异具显著统计学意义(P<0.01)。表明:光氧化反应处理的家兔胸主动脉在家兔胸-腹主动脉移植的动物模型中,生物稳定性高,细胞毒性低。

This study evaluated the biological stability and cytotoxicity of rabbit thoracic aorta cross-linked by dye-mediated photooxidation in vivo environment of blood flow. Rabbits, whose allogenic thoracic aorta was cross- linked by dye-mediated pbotooxidation （DMP, n=6） and glutaraldehyde （GA, n=6）, were in the DMP group and GA group, respectively; rabbits, whose homogenic abdominal aorta was orthotopic transplanted （OT, n=6）, were in the OT group. Then the donor arteries were transplanted into the position of the abdominal arteries of rabbits. After operation, the animals were fed for 1 month, then each of graft arteries was removed for observation. Biological stability was evaluated through histological analysis under optical microscope. Cytotoxicity was evaluated through ratio of coverage of endothelial cell （ES） under scanning-electron microscope. The results showed that no statistically significant difference was noted between DMP group and OT group （P〉0.05）3 however, there was a significant difference between DMP group and GA group （P〈0.01）. The stability index in DMP group was much higher than that in GA group. There was a statistically significant difference between DMP group and GA group （P〈0.01）. Ratio was much higher in DMP group than in GA group. In conclusion, the arteries cross-linked by dye-mediated photooxidation treatment appeared to have higher biological stability and lower cytotoxicity in rabbit allogenic transplation model.