一种用于生物芯片荧光标记的线性扩增技术

Fluorescent Linear Labelling Method for Biochip Applications

荧光线性标记技术（Fluorescent linear labelling method）是一种有效、单试管、单一扩增的双色标记技术。样品RNA通过逆转录酶作用合成双链的cDNA，再应用T7RNA聚合酶合成cRNA，同时加入Cy3和Cy5标记的CTP，从而实现对核酸样品的标记。由于荧光线性标记技术可以实现对初始样品50-100倍的扩增，并且线性度较好，因此在生物芯片实验的样品标记领域具有重要的应用价值。

Fluorescent Linear Labelling technique is an effective, single-tube and linear amplification method. The sample eDNA was synthesized from total RNA, and was labelled antisense cRNA from double-stranded eDNA with T7RNA. polymerase; it can he used for hybridization to oligonucleotide biochip. Fluorescent Linear Labelling Method can result in 50- to 100-fold higher degrees of amplification, and has been shown to retain information on transcript abundance, thus making it an efficient, robust technique for fluorescent labelling on biochip.