角膜缘干细胞的鉴定、筛选与培养的研究进展

Identification,Purification and Culture of Limbal Epithelial Stem Cells

角膜缘干细胞(Li mbal epithelial stemcell,LESC)对于研究角膜上皮和重建眼表均有非常重要的意义。而如何鉴定、筛选以及筛选后如何培养扩增LESC仍是目前困扰干细胞研究的瓶颈。以往对LESC的定位仍集中于寻找相关标志物如P63、ABCG2、整合素等;而分离纯化干细胞的方法也有多种,如流式细胞仪筛选法、快速吸附法、细胞大小筛选法等;体外培养扩增干细胞亦集中于拟造一个干细胞niche环境即干细胞龛培养扩增LESC。本文对目前LESC一些热点标志物、分离方法以及体外拟造niche环境的研究做一综述。

Limbal epithelial stem cell is extremely significant in the study of ocular surface reconstruction. But it still a problem to identify, purify and culture them. At present, the identification of limbal epithelial stem cell still depends on its associated markers such as P63, ABCG2, integrin, etc. And, there are many kinds of methods to purify the stem cells, e.g. using flow cytometry, attaching to extra-cellular matrix quickly, measuring cell size, etc. But none of them could isolate stem cells effectively. This review summarizes some hot limbal epithelial stem cell markers, purification methods and stem cell niche created methods in vitro, which may meet future research needs for further improvement of the tissue engineering cornea.