大豆种传南方菜豆花叶病毒和烟草环斑病毒的GICA-RT-PCR检测

Detection of southern bean mosaic virus and tobacco ringspot virus in soybean seed by GICA-RT-PCR

南方菜豆花叶病毒(south bean mosaic virus,SBMV)和烟草环斑病毒(tobacco ringspot virus,TRSV)是我国重要的进境检疫性有害生物,两种病毒均为种传病毒,可随大豆种子实现远距离传播。本研究以这两种病毒的大豆病种子为试材,先用胶体金免疫层析试纸条(GICA)检测,将检测完病毒后的胶体金免疫层析试纸条上的T线和C线剪下,再用RT-PCR的方法将T线和C线上捕获的病毒直接进行扩增,这种将血清学与分子生物学相结合起来所建立的GICA-RT-PCR检测方法,省去了烦琐的总RNA提取的环节,简化了病毒检测的步骤,提高了检测的灵敏度。

South bean mosaic virus （SBMV） and tobacco ringspot virus （TRSV） are quarantine pests for China. Both of them can be transmitted through soybean seeds. In this study, gold immunochromatography assay （ GICA） of both viruses was firstly prepared. After the GICA was used to detect the virus from seed, the T and C line of GICA were cut by scissors and applied to RT-PCR analysis. This GICA-RT-PCR method could simplify the routine RT-PCR detection steps, and greatly increased the sensitivity of GICA.