妇科活血止痛颗粒对血淤模型大鼠血液流变学的影响

Effect of Fukehuoxuezhitong Granule on Hemorheology in Acute Blood-stasis Model Rats

目的观察妇科活血止痛颗粒对急性血淤模型大鼠血液流变学的影响。方法雌性SD大鼠50只,随机分为空白对照组,急性血淤模型组,妇科活血止痛颗粒低剂量组(0.23g/kg)、中剂量组(0.46g/kg)和高剂量组(0.92g/kg),每组10只,全部动物以标准饲料喂养,3次/d,对照组和血淤模型组大鼠每日末次喂食后1h以10mL/kg生理盐水灌胃,低、中高剂量组每日末次喂食1h后按0.23、0.46、0.92g/kg剂量给予妇科活血止痛颗粒混悬液灌胃,灌胃容量10mL/kg,共10 d。第10天给药1 h后,除对照组外的其他组动物均皮下注射肾上腺素,冰水浸泡建造急性血淤模型,造模结束18h后,股动脉取血测定不同切变率的全血黏度、血浆黏度、红细胞压积、红细胞聚集指数等指标。结果血淤模型组大鼠全血黏度、全血还原黏度(低切和中切)明显高于对照组(P<0.05或P<0.01);低中剂量组明显低于血淤模型组(P<0.05或P<0.01);血淤模型组大鼠红细胞压积、红细胞聚集指数明显高于对照组(P<0.05或P<0.01),红细胞压积低、中、高剂量组大鼠均低于血淤模型组(P<0.05或P<0.01),红细胞聚集指数积低、中剂量组大鼠均低于血淤模型组(P<0.05或P<0.01)。结论妇科活血止痛颗粒对改善大鼠血液流变学状态具有良好作用。

Objective To observation the effect of Fukehuoxuezhitong granule on hemorheology in acute blood stasis model rats. Methods 50 female SD rats were randomly divided into five groups： normal control group, acute blood stasis model group, Fukehuoxuezhitong granule of low-dose group （0.23g/kg）, middle-dose group （0.46g/kg） and high-dose group （0.92g/kg）, all animals fed a standard diet, 3 times/d, the control group and blood stasis model rats fed to 10ml/kg intragastric administrated saline 1 h after the last day. low-, middle- and high-dose group fed 0.23,0.46,0.91g/kg doses of Fukehuoxuezhitong granule lh after, by gavage capacity 10mL/kg, a total of 10 d. 1 h after the last day, with the exception of the control group , other group of animals were made by soaked ice water after injected adrenaline hydrochloride in a major dose. The blood viscosity （high, middle, low shears）, blood re- duce viscosity, plasma viscosity, hematocrit and erythrocyte aggregation index were measured with Auto-Viscometer. Results Compared with control group, blood stasis model group showed middle,low shear viscosity increased sig- nificantly （ P 〈 0.05 or P 〈 0.01 ）, middle- and low-dose groups had a decline in the enhancement of whole blood viscosity in rats with blood stasis （P 〈0.05 or P 〈0.01）. the hematocrit and the erythrocyte aggregation index of the blood stasis model group Significantly increased as compared with control group（ P 〈 0. 05 or P 〈 0. 01）, In the low-, middle- and high-dose groups, the hematocrit and the erythrocyte aggregation index decreased （ P 〈 0. 05 or P 〈 0.01）. Conclusion Fukehuoxuezhitong granule has the fine effect to improving rat on hemorheology.