TNF-α和IL-6在腹部肠管火器伤后血清中的变化

Changes in Serum TNF-α and IL-6 after Intestinal Firearm Wounds in Pigs

目的探讨腹部肠管火器伤后血清肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)的动态变化规律,为寻找有效的细胞因子拮抗剂来减轻腹部火器伤后机体的损伤以促进机体康复提供理论依据。方法健康长白仔猪42头随机等分为对照组、伤后1、2、4、8、12 h和24 h组,每组6只,实验各组建立猪腹部肠管火器伤模型后,采用双抗体夹心ELISA法测定各组动物血清TNF-α、IL-6水平。结果实验组各组动物血清TNF-α、IL-6水平均明显高于对照组(P<0.05),TNF-α在伤后12 h为(94.36±10.18)ng/L,IL-6在伤后12 h为(1 218.35±74.00)ng/L,二者均于伤后12 h达到高峰。结论TNF-α、IL-6参与了腹部肠管火器伤后机体的病理生理过程,可能是引起腹部肠管火器伤后多器官功能障碍综合征(MODS)的重要因素之一。

Objective To investigate the dynamic changes in serum tumor necrosis factor-α （TNF-α） and interleukin-6 （IL-6） after intestinal firearm wounds in pigs so as to provide theoretical base for the search for effective antagonists against eytokines to pal. liate the injury and prompt rehabilitation. Methods A total of 42 Chang-Bai piglets were randomized into 7 groups ： control group and wounded 1-hour group, 2-hour group, 4-hour group, 8-hour group, 12-hour group and 24-hour group. The models of intestinal perforations due to abdominal firearm wounds were established in the wounded groups. Serum TNF-α and IL-6 levels were determined by ELISA. Results The levels of serum TNF-α and IL-6 levels in the wounded groups were significantly elevated compared to that of the control group （P 〈0.05）. At the time of 12 hours, the serum concentrations of TNF-α and IL-6 were （94.36±10.18） and （ 1 218.35±74.00） ng/L, both reaching their peaks. Conclusion TNF-α and IL-6, which participate in the pathophysiological process after intestinal firearm wounds, may be two of the important causes leading to multiple organ dysfunction syndrome （NODS） after intestinal firearm wounds.