梗阻性黄疸对大鼠疼痛行为学的影响

Changes in Pain-related Behaviors in Rats with Obstructive Jaundice

目的观察胆管结扎引起的阻塞性黄疸对大鼠疼痛行为学的影响。方法胆管结扎建立梗阻性黄疸大鼠模型.于术前及术后笫3、7、10天尾静脉取血测定血胆红素,采用热辐射法测定大鼠缩足潜伏期观察热疼痛阈值,Von Frey细丝法测定大鼠缩足反应百分比观察机械性疼痛阈值,从而研究黄疸大鼠疼痛行为学的变化。结果与假手术组大鼠相比较,阻塞性黄疸大鼠术后胆红素水平显著增高(P<0.0 5),热辐射引起的缩足潜伏期显著延长,Von Frey细丝机械性刺激引起的缩足反应百分比明显减少。结论梗阻性黄疸大鼠对热刺激和机械性刺激引起的疼痛反应不敏感,表现出明显的抗伤害性感受行为。

Objective To observe the effect of obstructive jaundice on pain-related behaviors in rats. Methods Obstructive jaundice rat models were produced by the ligation of bile duct. Their blood was obtained from the caudal veins for the assessment of bilirubin. Hargreaves test was applied and paw withdrawal latencies were recorded as a parameter of nociceptive response to thermal stimulation. Moreover, a yon Frey monofilament with bending forces of 9.9 g was applied to the middle of the paw for 10 times and percentage of withdrawal response was taken as a parameter of nociceptive response to mechanical stimulation. Results After the ligation of bile duct, increased paw withdrawal latency to thermal stimulation and decreased percentage of withdrawal response to mechanical stimulation were observed in the rats with obstructive jaundice compared with operated sham animals. Conclusion Obstructive jaundice causes the inerease in nociceptive threshold in response to both the thermal and mechanical stimulation in rats.