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大孔强酸性离子交换树脂填充EVAL中空纤维膜吸附剂对牛血红蛋白的吸附性能 被引量:2

Adsorption of bovine Hemoglobin on EVAL hollow fiber membrane filled with strongly acidic macroporous cation exchange resins
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摘要 以亲水性的乙烯-乙烯醇共聚物(EVAL)为中空纤维膜吸附剂的基质材料,以粉末型大孔强酸性阳离子交换树脂D061为功能性颗粒.采用干-湿法制备了不同填充量树脂填充EVAL中空纤维膜吸附剂,研究了这种吸附剂对模型物蛋白质牛血红蛋白(Hb)的吸附性能,考察了树脂填充量、蛋白质缓冲溶液的pH值和吸附时间对蛋白质静态吸附性能的影响.结果表明.阳离子树脂D061填充EVAL中空纤维膜吸附剂对蛋白质具有较大的静态吸附容量,随树脂的填充量增加膜吸附剂对Hb的吸附容量增加;当缓冲溶液的pH值低于Hb的等电点(6.8~7.1)时中空纤维膜吸附剂对蛋白质的吸附量较大,而当缓冲溶液的pH值高于Hb的等电点时膜吸附剂对蛋白质的吸附量较小:当树脂填充量为65%,并且缓冲溶液的pH值为2.0时树脂填充中空纤维膜吸附剂对Hb的静态吸附量可达到54.84mg/g. Hollow fiber membrane adsorbents filled with different loading resins are prepared through dry-wet spinning process, by using hydrophilic copolymer EVAL as the hollow fiber substrate and powder D061 type cation exchange resin as functional particles. The EVAL hollow fiber membrane adsorbents filled with resin is developed to study its adsorption behavior with bovine Hemoglobin (Hb) in this work. The effects of resinfilled content, pH value of buffer solution and adsorption time on protein static adsorption performance are discussed. The results show that EVAL hollow fiber membrane adsorbents filled with D061 type cation exchange resins have good adsorption capacity. The static protein absorption capacity increases with ionexchange resins loading. The larger protein capacity of membrane adsorbents is observed when the pH value of Hb buffer solution below the pI of the protein. However when the pH value of Hb buffer solution over the pI of the protein the smaller protein absorption capacity of membrane adsorbents is found. The static Hb adsorption capacity is 54.84 mg/g when D061 resin loading content is 65% at pH 2.0.
出处 《天津工业大学学报》 CAS 北大核心 2009年第2期10-13,共4页 Journal of Tiangong University
基金 国家自然科学基金资助项目(50473025) 天津市应用基础重点项目(043803911) 重质油国家重点实验室开放课题
关键词 乙烯-乙烯醇共聚物(EVAL) 大孔型离子交换树脂 中空纤维膜吸附剂 牛血红蛋白 静态吸附量 EVAL macroporous exchange resin hollow fiber membrane adsorbents Hb static adsorption capacity
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参考文献11

  • 1商振华,周良模.膜亲和色谱的现状、发展和应用[J].化学进展,1995,7(1):47-59. 被引量:9
  • 2张玉忠,肖长发.膜色谱技术的进展[J].天津工业大学学报,2004,23(4):94-97. 被引量:10
  • 3THOMMES J, KULA M R. Membrane chromatography-An integrative concept in the downstream [J]. Processing of Proteins Biotechnology Progress, 1995,11 : 357-367.
  • 4KLEIN E. Affinity membranes: a 10-year review[J]. Journal of Membrane Science, 2000, 179: 1-27.
  • 5CHARCOSSET C. Purification of proteins by membrane chromatography [J]. Journal of Chemical Technology and Biotechnology, 1998, 71:95-110.
  • 6GHOSH R. Protein separation using membrane chromatography: opportunities challenges [J]. Chromatography A, 2002, 952: 13-27.
  • 7ROPER D K, LIGHTFOOT E N. Separation of biomolecules using adsorptive membranes [J]. Chromatography A, 1995, 702: 3-26.
  • 8AVRAMESCU M E, GIRONES M, BORNEMAN Z, et al. Mixed-matrix membrane adsorbents for protein separation [J]. Journal of Chromatography A, 2003, 1006 : 171-183.
  • 9AVRAMESCU M E, SAGER W F C, MULDER M H V, et al. Preparation of ethylene vinylalcohol copolymer membranes suitable for ligand coupling in affinity separation [J]. Journal of Membrane Science,2002, 210: 155-173.
  • 10AVRAMESCU M E,WESSLLING M. Preparation of mixed matrix adsorber membranes for protein recovery [J]. Journal of Membrane Science, 2003, 218 : 219-233.

二级参考文献38

  • 1商振华,于亿年,郭为,周良模.膜亲和色谱用于胰蛋白酶抑制剂的分离[J].分析测试学报,1993,12(1):21-25. 被引量:9
  • 2商振华,周良模.膜亲和色谱的现状、发展和应用[J].化学进展,1995,7(1):47-59. 被引量:9
  • 3Tennikova T B, Svec F. High performance membrane chromatography: highly effcient separation method dorproteins in ion-exchange, hydrophobic interaction and reversed phase modes[J]. J Chromatogr, 1993, 646:279 - 288.
  • 4Dosio F, Arpiccos, Canevari S, et al. Single step purification of immunotoxins containing a high ionic charge ribosome-inactivating protein clavin by carboxymethyl high performance membrane chromatography[J]. J Chromatography A, 1999,830 (2): 329 - 335.
  • 5Thommes J, Kula M R. Membrane chromatography-an integrative concept in the downstream processing of proteins[J].Biotechnol Prog, 1995,11:357 - 367.
  • 6Suto S, Noriko U. Chemical crosslinking of hydroxypropyl cellulose and chitosan blend[J]. J Appl Polym Sci, 1996,61(13) :2 273 -2 278.
  • 7Platonova G A, Pankova G A, Ilina I Y, et al. Quantitative fast fraction of a pool of polyclonal antibodies by immunoaffnity membrane chromatography[J]. J Chromatography A,1999,852( 1 ) :129 - 140.
  • 8Zeng X F, Ruekenstein E. Maeroporous chitin affinity membrane for wheat germ agglutinin purincation from Wheat-germ[J]. J Membrane Sci, 1997,156(1) :97 -107.
  • 9Zusman I. Gel fiber glass membranes for affinity chromatography columns and their application to cancerdetection [J]. J Chromatogr B, 1998,715:297 - 306.
  • 10Castilho L R, Deckwer W D, Anspach F B. Influence of matrix activation and polymer coating on the purification of human IgG with protein A affinity membrane[J]. J Membrane Sci, 2000,172:269 - 277.

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