地塞米松对嘧啶亚硝脲诱导人脑胶质瘤细胞凋亡的影响

Effect of Dexamethasone on Apoptosis of Human Brain Glioma Cells Induced by Nimustine in Vitro

目的观察地塞米松(DEX)对嘧啶亚硝脲(ACNU)诱导的人脑胶质瘤细胞凋亡的影响。方法分别以ACNU、DEX、ACNU联合DEX,作用于体外培养的人脑胶质瘤细胞系SHG-4460h,通过细胞形态学及流式细胞仪分析检测细胞凋亡。结果①形态学观察:ACNU组、ACNU联合DEX组,大部分瘤细胞呈现细胞凋亡的形态学改变。而DEX组及正常对照组仅个别细胞出现上述形态改变。②荧光显微镜观察及流式细胞仪分析:ACNU组、ACNU联合DEX组有典型的凋亡峰,且其瘤细胞的凋亡率明显高于DEX组及正常对照组瘤细胞的凋亡率(P<0.01),但ACNU组的SHG-44细胞的凋亡率与ACNU联合DEX组相差不显著(P>0.05)。结论DEX对ACNU诱导人脑胶质瘤细胞凋亡没有明显影响。

Objectives To observe the effect of the Dexamethasone（DEX） on apoptosis of human brain gliomas cells induced by Nimustine hydrochloride （ACNU） in vitro. Methods Human malignant glioma cell lines SHG-44 cells, which were cultured, were exposed to ACNU, DEX and ACNU combined with DEX respectively for 60 hours in vitro. Then Apoptosis Rate of SHG-44 cells were determined by morphological technique and flow cytometry analysis. Results The morphological study showed that the apoptosis of most of SHG-44 cells occurred in both ACNU and ACNU combined DEX groups, and the apoptosis of only few SHG-44 cells occurred in DEX group. The flow cytometry analysis showed that the apoptosis rates of SHG-44 ceils in both the ACNU and ACNU combined with DEX groups, where the typical apoptotic peak was found, were significantly higher than those in DEX group and the normal control group （P〈0.01）. There was insignificant diference in the rate of the apoptosis of SHG-44 ceils between both the ACNU and ACNU combined DEX groups （P〉0.05）. Conclusion There was insignificant effect of DEX on apoptosis of SHG-44 cells induced by ACNU.