3′-大豆苷元磺酸钠对心肌缺血/再灌注损伤及抗氧化作用的影响

The therapeutic effects of 3′-daidzein sulfonate sodium on isolated myocardial ischemia/reperfusion injury and its relation with anti-oxidation

目的利用离体心脏灌流模型,研究3′-大豆苷元磺酸钠对离体心脏缺血/再灌注损伤的保护作用,并探讨其抗氧化作用的机制。方法应用Langendorff离体心肌缺血/再灌注损伤模型,在灌流液中加入高、中、低剂量的3′-大豆苷元磺酸钠,测定再灌注过程中冠脉流量、左心室收缩压的变化;比色法测定心肌组织中LDH、SOD、GSH-Px的活性。结果3′-大豆苷元磺酸钠可使缺血/再灌注后心脏灌流量及左心室收缩压增大,使心肌组织中LDH、SOD、GSH-Px的活性升高。结论3′-大豆苷元磺酸钠对离体心脏缺血/再灌注损伤具有保护作用,其保护机制与其增强抗氧化作用有关。

Aim To explore the therapeutic effects of 3＇-daidzein sulfonate sodium （DSS） on isolated myocardial ischemia/reperfusion injury and the relation of its mechanism to anti-oxidation. Methods An isolated rat myocardial ischemia/reperfusion injury was made, and the injury was treated with DSS. Following the treatment, the coronary blood flow levels, left ventricular systolic pressure, and activities of enzymes （ LDH, SOD, GSH-Px） were measured. Results After treat-merit, the isolated myocardial tissue showed an increase in the volume of perfusion and an increase in the enzymatic activities of LDH, SOD, GSH-Px in the myocardial tissue. Conclusion DSS has a therapeutic effect on isolated myocardial ischemia/reperfusion injury because it enhances anti-oxidative activity in myocardial tissue.