NF-κBp65、VEGF-C及受体VEGFR-3在乳腺癌组织中的表达

Expression and clinical significance of NF-κBp65、VEGF-C and VEGFR-3 in breast cancer

目的检测乳腺癌组织内NF-κBp65、VEGF-C及受体VEGFR-3的表达水平,并观察它们与临床病理特征的关系,为乳腺癌的早期诊断、治疗提供依据。方法采用免疫组化染色法检测50例乳腺癌组织及10癌旁组织内NF-κBp65、VEGF-C及受体VEGFR-3的表达,并分析与临床病理因素的关系。结果乳腺癌组织内NF-κBp65蛋白表达的阳性率为76.0%,明显高于癌旁对照组织30.0%,二者相比差异显著(P<0.05);乳腺癌组织内VEGF-C蛋白表达的阳性率为84.0%,明显高于癌旁对照组织20.0%,二者相比差异显著(P<0.05);乳腺癌组织内VEGFR-3蛋白表达的阳性率为88.0%,明显高于癌旁对照组VEGFR-3阳性表达率20.0%,两者相比差异显著(P<0.05);乳腺癌组织内NF-κBp65、VEGF-C及受体VEGFR-3三者的表达存在显著相关性(P<0.05),均与肿瘤淋巴转移密切相关(P<0.05),而与病人年龄、肿瘤大小无关(P>0.05)。结论乳腺癌中NF-κBp65的表达可能上调VEGF-C的表达,进而导致肿瘤周围淋巴管增生、扩张,促进肿瘤细胞向区域淋巴结转移。

Objective To promote early diagnosis and therapy of breast cancer, NF-KBp65,VEGF-C and VEGFR-3 in breast cancer were detected and their relationship with clinical factors were analysed. Methods Expression of NF-KBp65, VEGF-C and VEGFR-3 in 50 cases of breast cancer and 10 cases of normal tissue were detected by the immunohistochemical staining method. Results The expression of NF-KBp65 ,VEGF-C and VEGFR-3 were obviously higher in the cancer group than that of in the normal group（76.0%versus 30.0%, P〈0.05, respectively; 84.0%versus 20.0%, P〈0.05, Respectively; 88.0% versus 20.0%,P〈0.05,respectively）. Significant positive relationship was found between NF-KBp65 and VEGF-C, NF-KBp65 and VEGFR-3, VEGF-C and VEGFR-3 （P〈O.05）, and their expression was closely associated with lymphatic metastasis （P〈 0.05） but their expression was not associated with age and size of tumor （P〉0.05）. Conclusions NF-KBp65 can up-regulate VEGF-C expression in breast cancer, resulting in lymphangiogenesis and distension in the periphery of tumor, and then enhance tumor cells metastasize to the regional lymph nodes.