液态芯片技术在临床实验室诊断中的应用

Application of liquid chip technique in clinical laboratory diagnosis

液态芯片(liquid chip)是近年来新出现的一种临床应用型生物芯片,是以分类编码微球作为反应及信号检测载体,在液相反应体系中实现蛋白质、核酸等多种生物大分子的同步检测。既保持了生物芯片高通量的特性,又具有高灵敏度、高准确度、高精密度和线性测定范围宽及易操作等特点,在临床实验诊断领域具有广阔的应用前景。现就其基本原理、检测、特点在各临床诊断方面的应用作一综述。

Liquid chip,a new type of biochip classify encoding beads as carriers of reaction and signal detection,implement simultaneous determination protein,nucleic acid and other biomacromolecule in susp-ension liquid system.Apart from high-throughput characteristic as traditional biochip,the advantages of liquid chip are easy operation,the high-sensitivity,the high-accuracy,the high-precision and the great dynamic range and so on.It can be applied in the field of clinical laboratory diagnosis.In this review,we discuss the fundamental principle,detection,characteristics of liquid chip technique and the application in the field of clinical laboratory diagnosis.