摘要
以酵母菌株BWG1-7A基因组DNA为模板,利用PCR方法,扩增出耐盐基因Hal1,测序表明该基因全长为885个核苷酸,与已发表的序列NC_001148比较,同源性99.3%。将该基因插入表达载体pYES2的BamHI和EcoRI酶切位点之间,构建表达载体pYES2-Hal1,序列测定完全正确,为植物表达载体的构建打下基础。
Taking Saccharomyces cerevisiae BWG1-7A genomic DNA as template ,Hall gene is amplified by PCR.. The amplified product was digested with BamHl and EcoRI enzymes,then ligated into pYES2 vector. The recombinant pYES-Hal1 was successfully constructed and verified by DNA sequence,which provided a foundation of the construction of plant expression vector.
出处
《现代农业科技》
2009年第7期243-244,共2页
Modern Agricultural Science and Technology
